Medium pressure liquid chromatography and semipreparative high-performance liquid chromatography, followed by recycling high-performance liquid chromatography, were used for the isolation and purification of oligostilbenes from the acetone extracts ofDipterocarpus semivestitusandNeobalanocarpus heimii. Two stilbene dimers, ε-viniferin andcis-ε-viniferin, and a stilbene trimer,cis-miyabenol C were isolated fromD. semivestitus. A trimer, ampelopsin C, and two tetramers, hemsleyanol D and isohopeaphenol, were isolated fromN. heimii. The purities of the compounds were determined by an ultrahigh-performance liquid chromatography.