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- Title
Purification of Monomeric Prolactin Charge Isoform from Buffalo Pituitaries.
- Authors
Panchal, Manoj; Muralidhar, K.
- Abstract
A standard protocol for isolation of buffalo prolactin (buPRL) was modified at the alcohol precipitation step. This modification could separate lower molecular weight prolactin from the higher molecular weight prolactin (PRL). Reloading the prolactin onto a Sephacryl S-200 gel purified the buPRL monomer. The purity of buPRL monomer was confirmed by 15% SDS PAGE. The buPRL monomer was >90% pure. It was characterized by specific anti-buPRL serum in ELISA and Western blot. A native PAGE of the PRL showed three charge isoforms. A protocol was standardized to separate prolactin monomeric least acidic isoforms using an anion exchanger.
- Subjects
PROLACTIN; GONADOTROPIN; PITUITARY hormones; TRYPTOPHAN; AMINO acids; SULFUR
- Publication
Preparative Biochemistry & Biotechnology, 2008, Vol 38, Issue 1, p94
- ISSN
1082-6068
- Publication type
Academic Journal
- DOI
10.1080/10826060701774395