Results
Title

Preparation of a metal organic framework (MOF)-based monolithic column for the solid-phase extraction (SPE) of diosmetin from traditional Chinese medicine with determination by high-performance liquid chromatography (HPLC).

Authors

Wang, Shan; Han, Yamei; Chen, Jiangwei; Bai, Ligai; Yan, Hongyuan; Liu, Haiyan

Abstract

A metal organic framework (MOF)-based monolithic column was prepared by in situ polymerization using modified MIL-101-NH2 and glycidyl methacrylate (GMA) as monomers. The monolithic column was used as a solid phase extraction (SPE) adsorbent for on-line enrichment and preliminary purification of diosmetin in traditional Chinese medicine. After being characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption, the advantages of the monolithic column with MIL-101-NH2 added were reflected in the pore structure and specific surface area. The monolithic column for SPE had a linear relationship from 0.13 µg/mL to 5.0 × 102 µg/mL. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 µg/mL and 0.13 µg/mL, respectively. The recovery was from 96% to 104%, and the intra-day and inter-day relative standard deviations (RSDs) were below 1.1%. This method was suitable for the determination of diosmetin in complex samples.

Subjects

METAL-organic frameworks; HIGH performance liquid chromatography; CHINESE medicine; COLUMNS; GLYCIDYL methacrylate

Publication

Instrumentation Science & Technology, 2023, Vol 51, Issue 2, p222

ISSN

1073-9149

Publication type

Academic Journal

DOI

10.1080/10739149.2022.2120894