The effect of taraxerol acetate extracted from dandelion on alleviating oxidative stress responses in vitro.

Lu, Jiaquan; Yi, Siying; Wang, Shuna; Shang, Yafang; Yang, Shaohua; Cui, Kai

Oxidative stress can be alleviated by antioxidants intakes. Taraxerol acetate (TA), a natural triterpenoid extracted from dandelions, may reduce the risk of metabolic disorders by regulating oxidative stress. In the study, we investigated the effects of TA in relieving oxidative stress in murine intestinal epithelial cells using multiomics techniques. Here, we found that TA activated the antioxidant defense system. Total antioxidant capacity (T-AOC) and Catalase (CAT) activity notably increased after TA treatment. Additionally, TA treatment effectively reduced the levels of lactate dehydrogenase (LDH) and malonaldehyde (MDA) and alleviated H2O2-induced oxidative stress. Furthermore, TA induced significant changes in the levels of 30 important metabolites. Specifically, it activated the complement and coagulation cascades, NF-κB and MAPK and glycerophospholipid pathways, resulting in altered transcript levels of related genes, such as Serpinb9e, SCD2, Hspa1b, and Hspa1a. Thus, the results demonstrated that TA potentially could promote health by alleviating H2O2-induced oxidative damage and provide valuable insights for its further development.

OXIDANT status; OXIDATIVE stress; LACTATE dehydrogenase; TRANSCRIPTOMES; EPITHELIAL cells

Free Radical Research, 2024, Vol 58, Issue 12, p811

1071-5762

Academic Journal

10.1080/10715762.2024.2437640