Two triterpenoid glycosides from the roots of Camellia oleifera and their cytotoxic activity.

Yang, Ping; Li, Xia; Liu, Yan-Li; Xu, Qiong-Ming; Li, Yun-Qiu; Yang, Shi-Lin

Two new triterpenoid saponins, oleiferoside N and oleiferoside O, were isolated from the EtOH extract from the roots ofCamellia oleiferaC. Abel. Their structures were elucidated as 16α-acetoxy-21β,22α-O-diangeloyloxy-23,28-dihydroxyolean-12-ene 3β-O-β-d-xylopyranosyl-(1 → 3)-α-l-arabinopyranosyl-(1 → 3)-β-d-glucuronopyranoside (1), 16α-acetoxy-21β-O-angeloyloxy-23,28-dihydroxy-22α-O-(2-methylbutanoyloxy)olean-12-ene 3β-O-β-d-xylopyranosyl-(1 → 3)-α-l-arabinopyranosyl-(1 → 3)-β-d-glucuronopyranoside (2), on the basis of spectroscopic analyses (IR, ESI-MS, HR-ESI-MS, 1D and 2D NMR) and acid hydrolysis. Both were characterized to be oleanane-type saponins with sugar moieties linked to C-3 of the aglycone. Cytotoxic activities of two saponins were evaluated against four human tumor cell lines (A549, B16, BEL-7402, and MCF-7) by using the MTTin vitroassay. Compounds1and2showed moderate cytotoxic activities toward the tested cell lines.

ANIMAL experimentation; ANTINEOPLASTIC agents; BIOLOGICAL assay; CELL culture; CELL lines; CHROMATOGRAPHIC analysis; GLYCOSIDES; INFRARED spectroscopy; MASS spectrometry; MICE; NUCLEAR magnetic resonance spectroscopy; RESEARCH funding; PLANT roots; TERPENES; TOXICITY testing; DESCRIPTIVE statistics; IN vitro studies

Journal of Asian Natural Products Research, 2015, Vol 17, Issue 8, p800

1028-6020

Academic Journal

10.1080/10286020.2015.1004630