Results
Title

Alkaloids from the hook-bearing branch of Uncaria rhynchophylla and their neuroprotective effects against glutamate-induced HT22 cell death.

Authors

Qi, Wen; Yue, Si-Jia; Sun, Jia-Hong; Simpkins, James W.; Zhang, Lin; Yuan, Dan

Abstract

One new alkaloid, 4-geissoschizine N-oxide methyl ether (1), was isolated from the EtOH extract of the hook-bearing branch of Uncariarhynchophylla, together with 10 known alkaloids, 3-epi-geissoschizine methyl ether (2) isolated from U.rhynchophylla for the first time, geissoschizine methyl ether (3), 4-hirsuteine N-oxide (4), hirsuteine (5), hirsutine (6), 3α-dihydro-cadambine (7), 3β-isodihydro-cadambine (8), cadambine (9), strictosamide (10), and akuammigine (11). The structures were elucidated by spectroscopic methods including UV, ESI-QTOF MS, NMR, and circular dichroism experiments. Neuroprotective effects of 1–9 were investigated against 3 mM glutamate-induced HT22 cell death. The activity assay showed that 2, 3, 5, and 6 exhibited potent neuroprotective effects against glutamate-induced HT22 cell death. However, only weak neuroprotective activities were observed for 1, 4, 7, 8, and 9.

Subjects

ALKALOIDS; ANIMAL experimentation; BIOLOGICAL assay; CELL culture; CELL death; GLUTAMIC acid; MASS spectrometry; MICE; MOLECULAR structure; NUCLEAR magnetic resonance spectroscopy; PHARMACEUTICAL chemistry; RESEARCH funding; NEUROPROTECTIVE agents; DESCRIPTIVE statistics

Publication

Journal of Asian Natural Products Research, 2014, Vol 16, Issue 8, p876

ISSN

1028-6020

Publication type

Academic Journal

DOI

10.1080/10286020.2014.918109