Results
Title

Tetraclinis articulata essential oil emulsion use as alternative to chemical fungicide to control tomato grey mould disease.

Authors

Rguez, Safa; Hamrouni Sellami, Ibtissem; Abid, Ghassen; Papetti, Adele; Hammami, Majdi; Souissi, Fatma; Chaouachi, Manel; Muhovski, Yordan; Msaada, Kamel; Djébali, Naceur

Abstract

Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC50 of free radical-scavenging activity and H2O2 content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes.

Subjects

THERAPEUTIC use of antioxidants; THERAPEUTIC use of essential oils; PROTEINS; POLYPHENOLS; BIOLOGICAL products; PESTICIDES; PLANT diseases; EMULSIONS; FUNGI; SUPEROXIDE dismutase; PLANTS; DEFENSE mechanisms (Psychology); GLYCOSIDASES; TOXICITY testing; POLYMERASE chain reaction; TRANSCRIPTION factors; HYDROGEN peroxide

Publication

International Journal of Environmental Health Research, 2024, Vol 34, Issue 2, p1100

ISSN

0960-3123

Publication type

Academic Journal

DOI

10.1080/09603123.2023.2199973