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- Title
Histomorphometric analysis of the endometrium in an ectopic model of endometriosis in mice.
- Authors
da Silva, Felipe Rosendo; Soares Thimoteo, Daniel; Ferraz Carbonel, Adriana; Fuchs, Luiz Fernando Portugal; da Silva Sasso, Gisela Rodrigues; dos Santos Simões, Ricardo; Invitti, Adriana; Ramos Vieira, Renata; de Souza Ferreira, Luiz Philipe; da Silva, Rafael André; Lima, Patricia Daniele Azevedo; Soares Júnior, José Maria; de Jesus Simões, Manuel; Bertoncini, Clélia Rejane Antonio
- Abstract
Objective: Evaluate histomorphometry of ectopic and eutopic endometrial tissues in receptor mice. Method: Eighteen female Balb/c were divided into 3 groups, 6 animals each: GI Control, no procedure; GII – Sham, animals that had the same procedures as GIII without receiving the ectopic endometrial implant. Instead, they received saline solution; GIII – endometriosis model, animals had surgical intervention with an ectopic endometrial implant. GI and GIII mice were treated with 17β-estradiol, 100 µg/kg each. All animals were euthanized to collect uterine horns, which were fixed in 4% paraformaldehyde, embedded in paraffin, stained with Hematoxilin and Eosin and submitted to histomorphometric analyzes. Data underwent one-way ANOVA followed by Tukey's test. Results: Local tissue growth, showing important lesions and adhesions, as well as dark cysts were noticed. In GIII group, there was an increase in number of blood vessels and glands (GIII ≥ GI and GIII p >.001). Thickening of the GIII endometrial epithelial was also evident (GIII ≥ GI and GIII. p >.001). We also noticed an increase in the number of eosinophils (GIII (GIII ≥ GI and GIII. p >.001). Conclusion: Easy to perform model, capable of reproducing morphological endometriosis characteristics. From our findings, there was an increase of endometrial thickness as well as an increase in the eosinophils population.
- Subjects
ENDOMETRIUM; TUKEY'S test; SINUS augmentation; SALINE solutions; ONE-way analysis of variance
- Publication
Gynecological Endocrinology, 2022, Vol 38, Issue 10, p874
- ISSN
0951-3590
- Publication type
Academic Journal
- DOI
10.1080/09513590.2022.2119218