Chitosan oligosaccharide promotes osteoclast formation by stimulating the activation of MAPK and AKT signaling pathways.

Bai, Bing-Li; Xie, Zhong-Jie; Weng, She-Ji; Wu, Zong-Yi; Li, Hang; Tao, Zhou-Shan; Boodhun, Viraj; Yan, De-Yi; Shen, Zi-Jian; Tang, Jia-Hao; Yang, Lei

Chitosan Oligosaccharide (COS) has been widely used for the systemic treatment of clinical diseases such as bone tissue engineering. However, its influence on osteoclast formation, which plays a critical role in bone homeostasis, has never been investigated. The aim of this study was to investigate the effect of chitosan oligosaccharide on differentiation of osteoclast. Using cell counting kit-8, tartrate-resistant acid phosphatase staining, reverse transcription‑quantitative polymerase chain reaction assay and western blot analysis, we demonstrated that chitosan oligosaccharide cannot inhibit RANKL-induced osteoclast precursor proliferation but does promote osteoclast differentiation by stimulating the activation of p38/mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK)/MAPK, extracellular signal-regulated kinase (ERK)/MAPK and protein kinase B (AKT) without affecting nuclear factor kappaB (NF-kB) signaling pathways. Based on the promoting effect of chitosan oligosaccharide on osteoclast differentiation, we suggest that this property of chitosan oligosaccharide may have potential detrimental effect on bone homeostasis.

CHITOSAN; OLIGOSACCHARIDES; TISSUE engineering; OSTEOCLASTS; MITOGEN-activated protein kinases; THERAPEUTICS

Journal of Biomaterials Science -- Polymer Edition, 2018, Vol 29, Issue 10, p1207

0920-5063

Academic Journal

10.1080/09205063.2018.1448336