Results
Title

Antioxidant activity of phenylethanoid glycosides on glutamate-induced neurotoxicity.

Authors

Ji, Shi-Liang; Cao, Ke-Ke; Zhao, Xing-Xing; Kang, Nai-Xin; Zhang, Ying; Xu, Qiong-Ming; Yang, Shi-Lin; Liu, Yan-Li; Wang, Cheng

Abstract

Exposure of PC12 cells to 10 mM glutamate caused significant viability loss, cell apoptosis, decreased activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as increased levels of malondialdehyde (MDA). In parallel, glutamate significantly increased the intracellular levels of ROS and intracellular calcium. However, pretreatment of the cells with acteoside and isoacteoside significantly suppressed glutamate-induced cellular events. Moreover, acteoside and isoacteoside reduced the glutamate-induced increase of caspase-3 activity and also ameliorated the glutamate-induced Bcl-2/Bax ratio reduction in PC12 cells. Furthermore, acteoside and isoacteoside significantly inhibited glutamate-induced DNA damage. In the mouse model, acteoside significantly attenuated cognitive deficits in the Y maze test and attenuated neuronal damage of the hippocampal CA1 regions induced by glutamate. These data indicated that acteoside and isoacteoside play neuroprotective effects through anti-oxidative stress, anti-apoptosis, and maintenance of steady intracellular calcium. Protective effect of acteoside and isoacteoside on glutamate-induced cytotoxicity in PC12 cells.

Subjects

INTRACELLULAR calcium; MAZE tests; GLUTATHIONE peroxidase; NEUROTOXICOLOGY; SUPEROXIDE dismutase; GLYCOSIDES

Publication

Bioscience, Biotechnology & Biochemistry, 2019, Vol 83, Issue 11, p2016

ISSN

0916-8451

Publication type

Academic Journal

DOI

10.1080/09168451.2019.1637243