Localization of the CAPRICE-ENHANCER OF TRY AND CPC1 chimera protein in Arabidopsis root epidermis.

Tominaga-Wada, Rumi; Kurata, Tetsuya; Wada, Takuji

TheCAPRICE(CPC) encodes an R3-type MYB transcription factor, which promotes root-hair differentiation. Previously, we showed that the CPC protein moves from the non-hair cell to the neighboring cell and induces root-hair differentiation inArabidopsis. In addition, we proposed two cell-to-cell movement signal sequences, S1 and S2, in CPC. However, an S1:2xGFP:S2 chimera protein did not move between root epidermal cells. Here, we show that the S1 and S2 sequences do not confer cell-to-cell movement or nuclear localization ability to a GFP protein. TheENHANCER OF TRY AND CPC1(ETC1) gene encodes theCPChomolog R3 MYB; this protein does not possess cell-to-cell movement ability or the S1 sequence. To elucidate whether the S1 sequence can induce cell-to-cell movement ability in ETC1,CPCp:S1:ETC1:2xGFPwas constructed and introduced intoArabidopsis. Our results indicate that the addition of the S1 sequence was not sufficient for ETC1 to acquire cell-to-cell movement ability.

ARABIDOPSIS; CHIMERISM

Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 9, p1762

0916-8451

Academic Journal

10.1080/09168451.2017.1343120