Title: Synthesis of biotinylated keratan sulfate repeating disaccharides.
Authors: Naoko Takeda; Jun-ichi Tamura
Abstract: The article focuses on the synthesis of keratan sulfate, a chain of glycan, with disaccharide unit Galßl-4GlcNAcß. Topics discussed include conjugation of disaccharids with biotin through hydrophilic linkers, coupling of glycosyl acceptor with trimethylsilyl triflate (TMSOTf) and analysis of synthesis of keratan sulfate through silica gel chromatography, thin layer chromatography and nuclear magnetic resonance. An overview of the chemical compounds used in the process is also given.
Subjects: DISACCHARIDES; BIOCONJUGATES; HYDROPHILIC compounds; TRIMETHYLSILYL compounds; THIN layer chromatography; COUPLING reactions (Chemistry); GLYCANS; BIOTIN
Publication: Bioscience, Biotechnology & Biochemistry, 2014, Vol 78, Issue 1, p29
ISSN: 0916-8451
Publication type: Academic Journal
DOI: 10.1080/09168451.2014.877834

Synthesis of biotinylated keratan sulfate repeating disaccharides.

Naoko Takeda; Jun-ichi Tamura