Synthesis of nanoparticles with antibacterial properties is of great interest for the development of textiles finishing, in general, and cotton fabric, in particular. In this work, TiO2nanoparticles at different concentrations were synthesized using nitric acid as a reducing and stabilizing agent. Cotton fabrics were first impregnated, to a pick-up of 100%, in colloidal solutions containing carboxymethyl cellulose (CMC)/polyvinyl alcohol(PVA)/TiO2nanocomposites. After that, the coated fabrics were exposed to gamma irradiation to produce TiO2nanoparticles stabilized in cross-linked CMC/PVA hydrogel. The formation of TiO2nanoparticles was confirmed by ultra violet/visible (UV/vis) and transmission electron microscopy, and the particle size distribution of the nanoparticles was determined using the dynamic light scattering. The surface morphology of the finished fabrics was examined by scanning electron microscopy (SEM). The SEM micrographs revealed that TiO2nanoparticles were deposited on the surface of cotton fibers. The antibacterial activity of the treated fabrics loaded with TiO2nanoparticles was evaluated againstEscherichia coli(Gram -ve) bacteria.