Title: Preparation of a Novel Skeletal Porous Polymeric Monolith by Reverse Atom Transfer Radical Polymerization for Application to High Performance Liquid Chromatography.
Authors: Bai, Ligai; Yang, Gengliang; Gu, Yanzhao; Lei, Huan
Abstract: A novel polymeric monolith was synthesized by reverse atom transfer radical polymerization (R-ATRP) with I2being added in the polymerization as moderator. The polymeric monolith had skeletal, porous, and uniform structure. In this process, vinyl carboxylate was used as the monomer; 2,2′-azobisisobutyronitrile (AIBN) as initiator; and FeCl3as catalyst. The polymeric monolith was characterized by scanning electron microscopy, mercury proximate, and Fourier transform infrared spectroscopy. The prepared polymeric monolith was used as the high performance liquid chromatography (HPLC) stationary phase to successfully separate lysozyme from chicken egg white. Additionally, four standard proteins [bovine serum albumin (BSA), lysozyme, human immune globulin (IgG), and papain] were separated successfully by using the monolith.
Subjects: ADDITION polymerization; POROUS polymers; MONOLITHIC reactors; ATOM transfer reactions; REACTOR moderators; HIGH performance liquid chromatography; IMMUNOGLOBULIN G
Publication: Analytical Letters, 2013, Vol 46, Issue 1, p87
ISSN: 0003-2719
Publication type: Academic Journal
DOI: 10.1080/00032719.2012.704536

Preparation of a Novel Skeletal Porous Polymeric Monolith by Reverse Atom Transfer Radical Polymerization for Application to High Performance Liquid Chromatography.

Bai, Ligai; Yang, Gengliang; Gu, Yanzhao; Lei, Huan