GUTI: a new antigen in the Cromer blood group system.

Storry, Jill R.; Sausais, Laima; Kim Hue-Roye; Mudiwa, Flora; Ferre, Zennie; Blajchman, Morris A.; Lublin, Douglas M.; Bei-Wen Ma; Miquel, Juan F.; Nervi, Flavio; Pereira, Jaime; Reid, Marion E.; Hue-Roye, Kim; Ferrer, Zennie; Ma, Bei-Wen

Background: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI.Study Design and Methods: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI.Results: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4.Conclusion: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.

IMMUNE recognition; DNA

Transfusion, 2003, Vol 43, Issue 3, p340

0041-1132

Academic Journal

10.1046/j.1537-2995.2003.00319.x