5-Lipoxygenase Gene Expression in Hemodialysis.

Menegatti, Elisa; Roccatello, Dario; Rossi, Daniela; Formica, Marco; Piccoli, Giuseppe; Sena, Luigi M.

Leukotrienes (LTs), the end products of the eicosanoid pathway released during inflammation, are markers of polymorphonuclear cell and monocyte activation. The present study focused on the possibility that 5-lipoxygenase (5-LO), the key enzyme for LT synthesis, was involved in the interaction between blood and the hemodialysis (HD) membrane. 5-LO gene expression was examined by reverse transcriptase polymerase chain reaction (RT-PCR) in samples of mononuclear cells isolated from peripheral blood withdrawn at the start and at 15 min of HD from 10 chronic HD patients, 5 treated with Cuprophan and 5 with polymethylmethacrylate (PMMA) membrane. An increased 5-LO gene expression was detected at 15 min in 4 of 5 patients using the Cuprophan membrane but in none of the 5 PMMA treated patients. Our results showed for the first time that the interaction between blood and the HD membrane upregulates 5-LO messenger ribonucleic acid (mRNA).

LIPOXYGENASES; GENE expression

Artificial Organs, 1998, Vol 22, Issue 2, p140

0160-564X

Academic Journal

10.1046/j.1525-1594.1998.06047.x