Interaction of α-synuclein and synphilin-1: effect of Parkinson's disease-associated mutations.

Kawamata, Hibiki; McLean, Pamela J.; Sharma, Nutan; Hyman, Bradley T.

α-Synuclein is a major component of Lewy bodies, a neuropathological feature of Parkinson's disease. Two α-synuclein mutations, Ala53Thr and Ala30Pro, are associated with early onset, familial forms of the disease. Recently, synphilin-1, a protein found to interact with α-synuclein by yeast two hybrid techniques, was detected in Lewy bodies. In this study we report the interaction of α-synuclein and synphilin-1 in human neuroglioma cells using a sensitive fluorescence resonance energy transfer technique. We demonstrate that the C-terminus of α-synuclein is closely associated with the C-terminus of synphilin-1. A weak interaction occurs between the N-terminus of α-synuclein and synphilin-1. The familial Parkinson's disease associated mutations of α-synuclein (Ala53Thr and Ala30Pro) also demonstrate a strong interaction between their C-terminal regions and synphilin-1. However, compared with wild-type α-synuclein, significantly less energy transfer occurs between the C-terminus of Ala53Thr α-synuclein and synphilin-1, suggesting that the Ala53Thr mutation alters the conformation of α-synuclein in relation to synphilin-1.

LEWY body dementia; MICROBIAL mutation

Journal of Neurochemistry, 2001, Vol 77, Issue 3, p929

0022-3042

Academic Journal

10.1046/j.1471-4159.2001.00301.x