Title: False negative factor V Leiden assay following allogeneic stem cell transplant.
Authors: Crookston, Kendall P.; Henderson, Ruth; Chandler, Wayne L.
Abstract: We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality, whereas the initial genetic test (a PCR-based DNA assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cell transplant several months before, and the leucocytes in her blood were derived from the stem cell donor, which lacked the FV defect.
Subjects: GENES; TRANSPLANTATION immunology; CLASSIFICATION
Publication: British Journal of Haematology, 1998, Vol 100, Issue 3, p600
ISSN: 0007-1048
Publication type: Academic Journal
DOI: 10.1046/j.1365-2141.1998.00585.x

False negative factor V Leiden assay following allogeneic stem cell transplant.

Crookston, Kendall P.; Henderson, Ruth; Chandler, Wayne L.