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- Title
The different catalytic roles of the metal-binding ligands in human 4-hydroxyphenylpyruvate dioxygenase.
- Authors
Chih-Wei Huang; Hsiu-Chen Liu; Chia-Pei Shen; Yi-Tong Chen; Sung-Jai Lee; Lloyd, Matthew D.; Hwei-Jen Lee
- Abstract
4-Hydroxyphenylpyruvate dioxygenase (HPPD) is a non-haem iron(II)-dependent oxygenase that catalyses the conversion of 4- hydroxyphenylpyruvate (HPP) to homogentisate (HG). In the active site, a strictly conserved 2-His-1-Glu facial triad coordinates the iron ready for catalysis. Substitution of these residues resulted in about a 10-fold decrease in the metal binding affinity, as measured by isothermal titration calorimetry, and a large reduction in enzyme catalytic efficiencies. The present study revealed the vital role of the ligand Glu349 in enzyme function. Replacing this residue with alanine resulted in loss of activity. The E349G variant retained 5% activity for the coupled reaction, suggesting that co-ordinating water may be able to support activation of the trans-bound dioxygen upon substrate binding. The reaction catalysed by the H183A variant was fully uncoupled. H183A variant catalytic activity resulted in protein cleavage between Ile267 and Ala268 and the production of an N-terminal fragment. The H266A variant was able to produce 4-hydroxyphenylacetate (HPA), demonstrating that decarboxylation had occurred but that there was no subsequent product formation. Structural modelling of the variant enzyme with bound dioxygen revealed the rearrangement of the coordination environment and the dynamic behaviour of bound dioxygen in the H266A and H183A variants respectively. These models suggest that the residues regulate the geometry of the reactive oxygen intermediate during the oxidation reaction. The mutagenesis and structural simulation studies demonstrate the critical and unique role of each ligand in the function of HPPD, and which correlates with their respective co-ordination position.
- Subjects
CATALYSIS; LIGANDS (Biochemistry); CATALYTIC activity; ISOTHERMAL titration calorimetry; HEME oxygenase; OXYGENASES; TYROSINE metabolism
- Publication
Biochemical Journal, 2016, Vol 473, Issue 9, p1179
- ISSN
0264-6021
- Publication type
Academic Journal
- DOI
10.1042/BCJ20160146