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- Title
Human DC-SIGN binds specific human milk glycans.
- Authors
Noll, Alexander J.; Ying Yu; Lasanajak, Yi; Duska-McEwen, Geralyn; Buck, Rachael H.; Smith, David F.; Cummings, Richard D.
- Abstract
Human milk glycans (HMGs) are prebiotics, pathogen receptor decoys and regulators of host physiology and immune responses. Mechanistically, human lectins (glycan-binding proteins, hGBP) expressed by dendritic cells (DCs) are of major interest, as these cells directly contact HMGs. To explore such interactions, we screened many C-type lectins and sialic acid-binding immunoglobulin-like lectins (Siglecs) expressed by DCs for glycan binding on microarrays presenting over 200 HMGs. Unexpectedly, DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) showed robust binding to many HMGs, whereas other C-type lectins failed to bind, and Siglec-5 and Siglec-9 showed weak binding to a few glycans. By contrast, most hGBP bound to multiple glycans on other microarrays lacking HMGs. An α-linked fucose residue was characteristic of HMGs bound by DC-SIGN. Binding of DC-SIGN to the simple HMGs 2--fucosyl-lactose (2'-FL) and 3-fucosyl-lactose (3-FL) was confirmed by flow cytometry to beads conjugated with 2--FL or 3-FL, as well as the ability of the free glycans to inhibit DCSIGN binding. 2--FL had an IC50 of ∼1 mMfor DC-SIGN, which is within the physiological concentration of 2--FL in human milk. These results demonstrate that DC-SIGN among the many hGBP expressed by DCs binds to α-fucosylated HMGs, and suggest that such interactions may be important in influencing immune responses in the developing infant.
- Subjects
INTEGRINS; CELL adhesion molecules; DENDRITIC cells; GLYCANS; LECTINS
- Publication
Biochemical Journal, 2016, Vol 473, Issue 10, p1343
- ISSN
0264-6021
- Publication type
Academic Journal
- DOI
10.1042/BCJ20160046