Differential effects of selective oestrogen receptor modulators (SERMs) tamoxifen, ospemifene and raloxifene on human osteoclasts in vitro.

Michael, H.; Härkönen, P. L.; Kangas, L.; Väänänen, H. K.; Hentunen, T. A.

Background and purpose:Several selective oestrogen receptor modulators (SERMs) with oestrogen agonist effects in bone cells and without increased risk of breast and endometrial cancer have been developed. Here, we have investigated the effects of different types of SERMs on osteoclast differentiation, bone resorption and apoptosis in vitro.Experimental approach:Human peripheral blood-derived CD14 monocytes were cultured on bovine bone slices in the presence of RANKL, M-CSF, TNF-α and dexamethasone for seven days. Also, CD14 monocytes were co-cultured either with human SaOS-2 or MG-63 osteosarcoma cells, in the presence of parathyroid hormone. Osteoclast cultures were treated with different SERMs. TRACP multinucleated cells and C-terminal telopeptide of type I collagen were used as markers for osteoclast formation and bone resorption, respectively.Key Results:In CD14 monocyte cultures, tamoxifen directly inhibited human osteoclast formation and bone resorption, while raloxifene and ospemifene had no inhibitory effect. In the co-cultures either with SaOS-2 or MG-63 cells, ospemifene and raloxifene as well as tamoxifen inhibited osteoclast formation in a concentration-dependent manner. The inhibitory effect was associated with an increased production of osteoprotegerin. The anti-oestrogen ICI 182 780 completely reversed the effects of these SERMs.Conclusion and Implications:Tamoxifen had an oestrogen receptor dependent, direct, inhibitory effect on human osteoclast differentiation and bone resorption, whereas ospemifene and raloxifene required osteoblastic cells to achieve a similar inhibition. The effects of ospemifene and raloxifene were mediated by oestrogen receptors by a mechanism involving paracrine induction of osteoprotegerin in cultures with osteoblast derived osteosarcoma cells.British Journal of Pharmacology (2007) 151, 384–395; doi:10.1038/sj.bjp.0707232; published online 10 April 2007

SELECTIVE estrogen receptor modulators; ESTROGEN antagonists; TAMOXIFEN; RALOXIFENE; OSTEOCLASTS; BONE cells

British Journal of Pharmacology, 2007, Vol 151, Issue 3, p384

0007-1188

Academic Journal

10.1038/sj.bjp.0707232