The mitochondrial citrate carrier SLC25A1 regulates metabolic reprogramming and morphogenesis in the developing heart.

Ohanele, Chiemela; Peoples, Jessica N.; Karlstaedt, Anja; Geiger, Joshua T.; Gayle, Ashley D.; Ghazal, Nasab; Sohani, Fateemaa; Brown, Milton E.; Davis, Michael E.; Porter Jr., George A.; Faundez, Victor; Kwong, Jennifer Q.

The developing mammalian heart undergoes an important metabolic shift from glycolysis towards mitochondrial oxidation that is critical to support the increasing energetic demands of the maturing heart. Here, we describe a new mechanistic link between mitochondria and cardiac morphogenesis, uncovered by studying mitochondrial citrate carrier (SLC25A1) knockout mice. Slc25a1 null embryos displayed impaired growth, mitochondrial dysfunction and cardiac malformations that recapitulate the congenital heart defects observed in 22q11.2 deletion syndrome, a microdeletion disorder involving the SLC25A1 locus. Importantly, Slc25a1 heterozygous embryos, while overtly indistinguishable from wild type, exhibited an increased frequency of these defects, suggesting Slc25a1 haploinsuffiency and dose-dependent effects. Mechanistically, SLC25A1 may link mitochondria to transcriptional regulation of metabolism through epigenetic control of gene expression to promote metabolic remodeling in the developing heart. Collectively, this work positions SLC25A1 as a novel mitochondrial regulator of cardiac morphogenesis and metabolic maturation, and suggests a role in congenital heart disease. The mitochondrial citrate carrier SLC25A1 mediates key metabolic transitions during cardiac morphogenesis through epigenetic regulation of histone acetylation, ultimately supporting structural maturation of the embryonic heart.

DIGEORGE syndrome; CONGENITAL heart disease; METABOLIC reprogramming; METABOLIC regulation; HISTONE acetylation

Communications Biology, 2024, Vol 7, Issue 1, p1

2399-3642

Academic Journal

10.1038/s42003-024-07110-8