Degradation of 2,6-dicholorophenol by Trichoderma longibraciatum Isolated from an industrial Soil Sample in Dammam, Saudi Arabia.

Alabdalall, Amira H.; Aldakheel, Fatimah A.; Ababutain, Ibtisam M.; Chakroun, Hanen; Alghamdi, Azzah I.; Hammami, Ines; Al Dosary, Sahar K.; Youssef, Tamer E.; Albarrag, Ahmed M.; Aldakeel, Sumayh A.; Aldughaish, Rawan; Al Qurin, Nada; ElKomy, Hesham M.

2,6-Dichlorophenol (2,6-DCP) is an aromatic compound with industrial importance in making insecticides, herbicides, and other organic compounds. However, it poses serious health and ecological problems. Microbial degradation of 2,6-DCP has been widely applied due to its effectiveness and eco-friendly characteristics. In this study, Trichoderma longibraciatum was isolated from an industrial soil sample in Dammam, Saudi Arabia using the enrichment method of mineral salt's medium (MSM) amended with 2,6-DCP. Morphological and molecular identification (using the internal transcribed spacer rRNA gene sequencing) of the 2,6-DCP tolerating fungal isolate were charactraized. The fungal isolate has demonstrated a tolerance to 2,6-DCP up to 300 mg/L. Mycelial growth and fungal sporulation were reduced with increasing 2,6-DCP concentrations up to 96 h incubation period. However, after 168 h incubation period, the fungal isolate recorded maximum growth at all the tested 2,6-DCP concentrations up to 150 mg/L. Carboxy methyl cellulase production by tested fungus was decreased by increasing 2,6-DCP concentration up to 75 mg/L. The biodegradation pattern of 2,6-DCP in GM liquid medium using GC–mass analysis as well as the degradation pathway was presented. This study provides a promising fungal isolate that could be used in the bioremediation process for chlorinated phenols in soil.

DAMMAM (Saudi Arabia); SOIL sampling; FUNGAL spores; TRICHODERMA; ENVIRONMENTAL health; ORGANIC compounds

Scientific Reports, 2022, Vol 12, Issue 1, p1

2045-2322

Academic Journal

10.1038/s41598-022-07016-7