Results
Title

A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice.

Authors

Adunphatcharaphon, Saowalak; Kolawole, Oluwatobi; Sooksimuang, Thanasat; Panchan, Waraporn; Wasuthep, Wannee; Petdum, Anuwut; Pichayawaytin, Grit; Jintamethasawat, Rungroj; Doljirapisit, Narusorn; Somboonkaew, Armote; Noppakuadrittidej, Prae; Kaew-amdee, Sudtida; Makornwattana, Manlika; Meneely, Julie; Elliott, Christopher T.; Petchkongkaew, Awanwee; Karoonuthaisiri, Nitsara

Abstract

Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.

Subjects

FOOD safety; DETECTION limit; ORGANIC dyes; STANDARD deviations; IMMUNOASSAY; MYCOTOXINS; FUSARIUM toxins

Publication

NPJ Science of Food, 2024, Vol 8, Issue 1, p1

ISSN

2396-8370

Publication type

Academic Journal

DOI

10.1038/s41538-024-00342-2