Results
Title

Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9.

Authors

Martin, Gregory M.; Fernández-Quintero, Monica L.; Lee, Wen-Hsin; Pholcharee, Tossapol; Eshun-Wilson, Lisa; Liedl, Klaus R.; Pancera, Marie; Seder, Robert A.; Wilson, Ian A.; Ward, Andrew B.

Abstract

A primary objective in malaria vaccine design is the generation of high-quality antibody responses against the circumsporozoite protein of the malaria parasite, Plasmodium falciparum (PfCSP). To enable rational antigen design, we solved a cryo-EM structure of the highly potent anti-PfCSP antibody L9 in complex with recombinant PfCSP. We found that L9 Fab binds multivalently to the minor (NPNV) repeat domain, which is stabilized by a unique set of affinity-matured homotypic, antibody-antibody contacts. Molecular dynamics simulations revealed a critical role of the L9 light chain in integrity of the homotypic interface, which likely impacts PfCSP affinity and protective efficacy. These findings reveal the molecular mechanism of the unique NPNV selectivity of L9 and emphasize the importance of anti-homotypic affinity maturation in protective immunity against P. falciparum. The cryo-EM structure of the highly potent malaria antibody L9 reveals a key role of light-chain derived homotypic interactions in antigen binding and parasite inhibition, enabling antibody engineering and next-generation malaria vaccine design.

Subjects

IMMUNOGLOBULINS; PARASITE antigens; MONOCLONAL antibodies; CIRCUMSPOROZOITE protein; MALARIA; MALARIA vaccines; MOLECULAR dynamics

Publication

Nature Communications, 2023, Vol 14, Issue 1, p1

ISSN

2041-1723

Publication type

Academic Journal

DOI

10.1038/s41467-023-38509-2