In vivo biocompatibility of a new cyanine dye for ILM peeling.

Thaler, S; Haritoglou, C; Schuettauf, F; Choragiewicz, T; May, C A; Gekeler, F; Fischer, M D; Langhals, H; Schatz, A

PurposeTo investigate the biocompatibility of the new cyanine dye: 3,3′-Di-(4-sulfobutyl)-1,1,1′,1′-tetramethyl-di-1H-benz[e]indocarbocyanine (DSS) as a vital dye for intraocular application in an in vivo rat model and to evaluate the effects of this dye on retinal structure and function.MethodsDSS at a concentration of 0.5% was applied via intravitreal injections to adult Brown Norway rats with BSS serving as a control. Retinal toxicity was assessed 7 days later by means of retinal ganglion cell (RGC) counts, light microscopy, optical coherence tomography (OCT), and electroretinography (ERG).ResultsNo significant decrease in RGC numbers was observed. No structural changes of the central retina were observed either in vivo (OCT) or under light microscopy. ERGs detected a temporary reduction of retinal function 7 days after injection; this was no longer evident 14 days after injection.ConclusionsDSS showed good biocompatibility in a well-established experimental in vivo setting and may be usable for intraocular surgery as an alternative to other cyanine dyes. In contrast to indocyanine green, it additionally offers fluorescence in the visual spectrum. Further studies with other animal models are needed before translation into clinical application.

BIOCOMPATIBILITY; CYANINES; INTRAOCULAR drug administration; RETINA; RETINAL ganglion cells; TOXICITY testing

Eye (0950-222X), 2015, Vol 29, Issue 3, p428

0950-222X

Academic Journal

10.1038/eye.2014.295