Results
Title

Exoproteinases of the Oomycete Phytophthora infestans.

Authors

Gvozdeva, E. L.; Ievleva, E. V.; Gerasimova, N. G.; Ozeretskovskaya, O. L.; Valueva, T. A.

Abstract

When grown in a medium containing heat-stable potato tuber proteins, the oomycete Phytophthora infestans (Mont.) de Bary produces a set of exoproteinases active at neutral and mildly basic pH values. These extracellular proteinases have been shown (by SDS-PAGE in the presence of gelatin) to include at least six components differing in molecular weight. Inhibitory analysis and studies of the effects of the enzymes on various synthetic substrates show that the culture liquid of P. infestans contains mainly serine proteinases (specific for trypsin and subtilisin) and metalloproteinases. Their activity is suppressed by proteinase-inhibiting proteins from potato tubers. It is suggested that exoproteinases of P. infestans may be the metabolic target for natural proteinase inhibitors from potato.

Subjects

OOMYCETES; PHYTOPHTHORA infestans; PLANT proteins; POTATO genetics; LATE blight of potato; PROTEINS; PHYCOMYCETES

Publication

Applied Biochemistry & Microbiology, 2004, Vol 40, Issue 2, p165

ISSN

0003-6838

Publication type

Academic Journal

DOI

10.1023/B:ABIM.0000018920.38585.60