Characterization and regulation of NADP -isocitrate dehydrogenase from Saccharopolyspora erythraea.

Alvarado, Alejandra; Flores, María Elena

NADP+-Isocitrate dehydrogenase (ICDH) activity was detected in cell-free extracts of Saccharopolyspora erythraea CA340, an erythromycin producer. Apparent Km values for dl-isocitrate and NADP+ were 0.14 μM and 0.026 μM, respectively. ATP, ADP, GTP, citric acid, oxaloacetate, α-ketoglutarate, glyoxalate and glyoxalate plus oxaloacetate, each at 1 mM concentration, caused 50, 20 10, 50, 25, 60, 20 and 50% inhibition of ICDH activity, respectively. Phosphoenolpyruvate, fructose 1,6-diphosphate and pyruvate had no effect. ICDH specific activity profile was growth-associated and activity with dextrose or fructose as sole carbon source, was twice of that obtained with lactose.

DEHYDROGENASES; MICROBIAL enzymes; BACTERIA; SACCHARIDES; ERYTHROMYCIN; ANTIBACTERIAL agents; MACROLIDE antibiotics

Biotechnology Letters, 2003, Vol 25, Issue 14, p1175

0141-5492

Academic Journal

10.1023/A:1024542426956