Application of statistically-based experimental designs for the optimization of nisin production from whey.

Chuanbin Liu; Yan Liu; Wei Liao; Zhiyou Wen; Shulin Chen

Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l-1 for yeast extract, 0.569 g l-1 for KH2PO4, and 0.572 g l-1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment.

NISIN; ANTIBIOTICS; WHEY products; BIOSYNTHESIS; BIOCHEMICAL engineering; LACTOCOCCUS lactis

Biotechnology Letters, 2003, Vol 25, Issue 11, p877

0141-5492

Academic Journal

10.1023/A:1024009027255