Microbial transformation of ginsenoside Rb₁ by Rhizopus stolonifer and Curvularia lunata.

Aling Dong; Min Ye; Hongzhu Guo; Junhua Zheng; Dean Guo

Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[β-d-glucopyranosyl-(1,2)-β-d-glucopyranosyl]- 20-O-[β-d-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ene (1), 3-O-[β-d-glucopyranosyl-(1,2)-β-d- glucopyranosyl]-20-O-[β-d-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ol (2), 3-O-[β-d-gluco- pyranosyl-(1,2)-β-d-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (3), and 3-O-β-d-glucopyranosyl-3β, 12β, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

SAPONINS; METABOLITES; BIOTRANSFORMATION (Metabolism); PHARMACOLOGY; MASS spectrometry; NUCLEAR magnetic resonance spectroscopy

Biotechnology Letters, 2003, Vol 25, Issue 4, p339

0141-5492

Academic Journal

10.1023/A:1022320824000