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Title

Comparative Analysis of Proteome Maps of Helicobacter pylori Clinical Isolates.

Authors

Govorun, V. M.; Moshkovskii, S. A.; Tikhonova, O. V.; Goufman, E. I.; Serebryakova, M. V.; Momynaliev, K. T.; Lokhov, P. G.; Khryapova, E. V.; Kudryavtseva, L. V.; Smirnova, O. V.; Toropyguine, I. Yu.; Maksimov, B. I.; Archakov, A. I.

Abstract

The gram-negative bacterium Helicobacter pylori is found in human gastric mucosa. A widely distributed H. pylori infection is associated with chronic gastritis, gastric and duodenal ulcers, and malignant neoplasms. In this study proteome maps of four H. pylori clinical isolates derived from patients of two Russian regions (Moscow/Moscow Region and Novosibirsk) were obtained using 2D-electrophoresis and MALDI-TOF-mass-spectrometry. Variability of some H. pylori proteins and the level of their expression have been evaluated. These four isolates could be easily subdivided into two equal groups characterized by the close proteome profiles and the isolate from Moscow Region and the isolate from Novosibirsk constituted one group. The present study demonstrates the potential of proteome technology, which can be employed together with genome and transcriptome studies for the multiparameter typing of clinical isolates of pathogenic microorganisms.

Subjects

COMPARATIVE studies; HELICOBACTER pylori; TWO-dimensional electrophoresis; MASS spectrometry; PROTEOMICS; PATHOGENIC microorganisms; BIOCHEMISTRY

Publication

Biochemistry (00062979), 2003, Vol 68, Issue 1, p42

ISSN

0006-2979

Publication type

Academic Journal

DOI

10.1023/A:1022189200944

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