Results
Title

High density cell culture of Panax notoginseng for production of ginseng saponin and polysaccharide in an airlift bioreactor.

Authors

Han, Jin; Zhong, Jian-Jiang

Abstract

High cell density of Panax notoginseng in a 17 l airlift bioreactor was achieved in batch cultivation using a modified MS medium. The dry cell weight, ginseng saponin and polysaccharide reached 24, 1.7 and 2.8 g l-1, respectively, after 15 d. A strategy of sucrose feeding based on changes in the specific O2 uptake rate was applied to the cell cultures, which increased these respective yields to 30, 2.3 and 3.2 g l-1.

Subjects

CELL culture; SUCROSE; SAPONINS; GINSENG; DISACCHARIDES; CYTOLOGICAL techniques

Publication

Biotechnology Letters, 2002, Vol 24, Issue 22, p1927

ISSN

0141-5492

Publication type

Academic Journal

DOI

10.1023/A:1020960410885