Exopolysaccharide biosynthesis and related enzyme activities of the medicinal fungus, Ganoderma lucidum, grown on lactose in a bioreactor.

Tang, Ya-Jie; Zhong, Jiang-Jiang

Exopolysaccharide (EPS) production and biosynthesis were studied in Ganoderma lucidum, a fungus used in traditional Chinese medicine, grown with lactose in a bioreactor. β-Galactosidase activity, which implies the existence of a lactose permease system, was induced by lactose. Lactose feeding also increased α-phosphoglucomutase activity and EPS accumulation but decreased phosphoglucose isomerase activity and lactate concentration in the culture broth. A maximum cell density of 22 g l-1 and EPS at 1.25 g l-1 were obtained in fed-batch bioreactor culture.

LACTOSE; DISACCHARIDES; MEDICAL botany; BIOCHEMISTRY; MEDICINAL plants; ENZYMES

Biotechnology Letters, 2002, Vol 24, Issue 12, p1023

0141-5492

Academic Journal

10.1023/A:1015677313598