Cryopreservation of hairy root cultures of Vinca minor (L.) by encapsulation-dehydration.

Hirata, Kazumasa; Mukai, Misuzu; Goda, Saeko; Ishio-Kinugasa, Masumi; Yoshida, Kenji; Sakai, Akira; Miyamoto, Kazuhisa

Hairy root cultures of Vinca minor and Ajuga reptans var. atropurpurea could be cryopreserved when the roots were precultured and encapsulated in 2% (w/v) alginate beads with 0.3 M sucrose and 0.5 M glycerol and dehydrated until the bead weight reached 25% of the initial weight before cooling in liquid nitrogen. Preculture and encapsulation of the roots with abscisic acid was effective in increasing the survival rates. For V. minor root tips moreover a sufficiently high survival rate of more than 70% was attained by eliminating glycerol from the preculture medium and dehydration of beads until 23% of the initial weight was reached instead of 25%.

CULTURES (Biology); VINCA; ALGINATES; ABSCISIC acid; PLANT hormones; LIQUID nitrogen

Biotechnology Letters, 2002, Vol 24, Issue 5, p371

0141-5492

Academic Journal

10.1023/A:1014564804048