The Immune Response to Artificial Proteins Containing Biologically Active Fragments of Human IFN-α2 and Insulin.

Bocharova, O. V.; Moshkovskii, S. A.; Chertkova, R. V.; Kolesanova, E. F.; Dolgikh, D. A.; Kirpichnikov, M. P.

A study was made of the humoral immune response of BALB/c mice to various doses of artificial proteins that contained biologically active fragments of human interferon α2 (IFN-α2) and insulin. The insulin fragment had no effect on the response to any protein construct. The IFN-α2 fragment increased the titer of antibodies against the construct. Mapping of continuous B epitopes with immune sera revealed several antigenic determinants, the C end of the IFN-α2 fragment with the adjacent de novo protein region being immunodominant. The more effective binding of serum antibodies with the constructs containing the IFN-α2 fragment was attributed to antibody interaction with the fragment and to better recognition of the entire protein construct by the immune system.

BLOOD plasma; IMMUNOLOGY; HYPOGLYCEMIC agents; IMMUNOGLOBULINS; IMMUNE serums; ANTILYMPHOCYTIC serum

Molecular Biology, 2002, Vol 36, Issue 1, p65

0026-8933

Academic Journal

10.1023/A:1014298507314