Factorial design and response surface optimization of crude violacein for Chromobacterium violaceum production.

Mendes, Armando S.; de Carvalho, João E.; Duarte, Marta C. T.; Durán, Nelson; Bruns, Roy E.

Initially an eleven variable, sixteen assay 215-11 fractional factorial design, was used to determine the key factors in the production of violacein produced by Chromobacterium violaceum, CCT 3496. Subsequently five and three factor central composite designs were executed to determine response surfaces with the aim of optimizing cellular mass and crude violacein production. The 7.5 g l-1 dry cell mass and 0.17 g l-1 crude violacein productions obtained with our initial culture medium were increased to 21 g l-1 and 0.43 g l-1, respectively, for a medium investigated in the three factor design.

CHROMOBACTERIUM violaceum; RHIZOBIACEAE; GRAPHIC methods in statistics; ANALYSIS of variance; RESPONSE surfaces (Statistics); ASTERACEAE

Biotechnology Letters, 2001, Vol 23, Issue 23, p1963

0141-5492

Academic Journal

10.1023/A:1013734315525