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Title

Production of fructosylxyloside by the new fructosyltransferase from Bacillus macerans EG-6.

Authors

Nam, Soo-Wan; Yun, Hee-Jung; Ahn, Jung-Hee; Kim, Kwang-Hyun; Kim, Byung-Woo

Abstract

The new fructosyltransferase (FTase) from Bacillus macerans EG-6 showed a broad acceptor specificity, and resulted in the formation of fructosylxyloside (FX) with d-xylose being the most effective acceptor. The optimal FTase concentration for FX production was 0.6 unit per g sucrose, which gave the highest transfer ratio, 83%, of fructosyl moiety from sucrose to d-xylose. Maximum yield of FX was 114 g l-1 with 200 g sucrose l-1 and 300 g d-xylose l-1.

Subjects

BACILLUS (Bacteria); SUCROSE; BACILLACEAE; SUGARS; DISACCHARIDES; FRUCTOSE

Publication

Biotechnology Letters, 2000, Vol 22, Issue 15, p1243

ISSN

0141-5492

Publication type

Academic Journal

DOI

10.1023/A:1005665400590

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