A mixed anaerobic bacterial culture decolorized Drimaren Orange K-GL, Everzol Red RBN and Everdirect Supra Yellow PG dyestuffs at 200 mg dyestuff l-1 over 24 h. Improved performance with complete decolorization within 24 h was achieved by incubation with 5 g yeast extract l-1 compared to glucose, lactose and sucrose though 50 mg yeast extract l-1 supplemented with 5 g lactose l-1 or 5 g sucrose l-1 also resulted in complete decolorization within 24 h.