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- Title
Production of lactobionic acid from whey by Pseudomonas sp. LS13-1.
- Authors
Miyamoto, Yasumitsu; Ooi, Toshihiko; Kinoshita, Shinnichi
- Abstract
Pseudomonas sp. LS13-1 was isolated as a producer of lactobionic acid from whey and when grown with 207 g whey l-1 (150 g lactose l-1 equivalent) and three intermittent additions of 69 g whey l-1 (50 g lactose l-1 equivalent) in a fed-batch culture at pH 5.5 in a 2-l jar fermenter, it produced 175 g lactobionic acid l-1 after 180 h. In a lactose medium it produced 240 lactobionic acid l-1 from a total of 300 g lactose l-1 after 155 h. With the addition of 20 CaCO3 l-1 instead of pH control, 290 g lactobionic acid l-1 was produced in the lactose medium after 155 h with a yield of higher than 90% (mon mol-1).
- Subjects
PSEUDOMONAS; PSEUDOMONADACEAE; LACTOSE; CELL culture; FERMENTATION; DISACCHARIDES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 5, p427
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1023/A:1005617903152