Production of lactobionic acid from whey by Pseudomonas sp. LS13-1.

Miyamoto, Yasumitsu; Ooi, Toshihiko; Kinoshita, Shinnichi

Pseudomonas sp. LS13-1 was isolated as a producer of lactobionic acid from whey and when grown with 207 g whey l-1 (150 g lactose l-1 equivalent) and three intermittent additions of 69 g whey l-1 (50 g lactose l-1 equivalent) in a fed-batch culture at pH 5.5 in a 2-l jar fermenter, it produced 175 g lactobionic acid l-1 after 180 h. In a lactose medium it produced 240 lactobionic acid l-1 from a total of 300 g lactose l-1 after 155 h. With the addition of 20 CaCO3 l-1 instead of pH control, 290 g lactobionic acid l-1 was produced in the lactose medium after 155 h with a yield of higher than 90% (mon mol-1).

PSEUDOMONAS; PSEUDOMONADACEAE; LACTOSE; CELL culture; FERMENTATION; DISACCHARIDES

Biotechnology Letters, 2000, Vol 22, Issue 5, p427

0141-5492

Academic Journal

10.1023/A:1005617903152