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- Title
Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli.
- Authors
Won, Misun; Kim, Soyoun; Lee, Seunghwan; Kim, Chuljung; Kim, Hyunsu; Jun, Moohyung; Song, Kyung Bin
- Abstract
To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.
- Subjects
SALMONELLA; ENTEROBACTERIACEAE; PILI (Microbiology); BACTERIA morphology; CARRIER proteins; MALTOSE; DISACCHARIDES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 14, p1165
- ISSN
0141-5492
- Publication type
Academic Journal
- DOI
10.1023/A:1005605610004