Title: Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli.
Authors: Won, Misun; Kim, Soyoun; Lee, Seunghwan; Kim, Chuljung; Kim, Hyunsu; Jun, Moohyung; Song, Kyung Bin
Abstract: To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.
Subjects: SALMONELLA; ENTEROBACTERIACEAE; PILI (Microbiology); BACTERIA morphology; CARRIER proteins; MALTOSE; DISACCHARIDES
Publication: Biotechnology Letters, 2000, Vol 22, Issue 14, p1165
ISSN: 0141-5492
Publication type: Academic Journal
DOI: 10.1023/A:1005605610004

Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli.

Won, Misun; Kim, Soyoun; Lee, Seunghwan; Kim, Chuljung; Kim, Hyunsu; Jun, Moohyung; Song, Kyung Bin