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- Title
Effects of carbon monoxide (CO) delivery by a CO donor or hemoglobin on vascular hypoxia inducible factor 1α and mitochondrial respiration
- Authors
Reiter, Chad E.N.; Alayash, Abdu I.
- Abstract
Abstract: We examined carbon monoxide (CO) delivery by carbon monoxide-releasing molecule 2 (CORM-2) or hemoglobin (Hb) on cellular oxygen sensing and mitochondrial respiration in bovine aortic endothelial cells (BAECs). CORM-2 reduced hypoxia-inducible factor-1α (HIF-1α) and endothelin-1 (ET-1) expression in normoxic and hypoxic cells, but while Hb alone significantly reduced HIF-1α stabilization in hypoxic cells, CO delivered by Hb (Hb-CO) had no effect on HIF-1α stabilization. CO dose-dependently increased basal oxygen consumption and reduced overall mitochondrial respiratory capacity. Hb-CO increased basal oxygen consumption but did not alter respiratory capacity. Together, CO reduced ET-1, and, at low doses, had no effect on endothelial mitochondria oxygen consumption. CO ligation to Hb may be developed further as non-vasoactive oxygen therapeutic without compromising mitochondrial function.
- Subjects
CARBON monoxide; HEMOGLOBINS; HYPOXIA-inducible factor 1; ENDOTHELIAL cells; ENDOTHELINS; OXYGEN consumption; PROLINE hydroxylase
- Publication
FEBS Open Bio, 2012, Vol 2, p113
- ISSN
2211-5463
- Publication type
Academic Journal
- DOI
10.1016/j.fob.2012.05.003