Results
Title

Involvement of Na<sup> </sup>/H<sup> </sup> exchangers in induction of cyclooxygenase-2 by vacuolar-type (H<sup> </sup>)-ATPase inhibitors in RAW 264 cells

Authors

Kamachi, Fumitaka; Yanai, Maiko; Ban, Hyun Seung; Ishihara, Kenji; Hong, JangJa; Ohuchi, Kazuo; Hirasawa, Noriyasu

Abstract

Abstract: In the mouse macrophage-like cell line RAW 264, vacuolar-type (H )-ATPase (V-ATPase) inhibitors, bafilomycin A1 and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) and nuclear factor-κB, and by inhibitors of Na /H exchangers (NHEs). The bafilomycin A1-induced activation of JNK but not degradation of IκB-α was suppressed by NHE inhibitors and by an inhibitor of Na /Ca2 exchanger SN-6. These results suggested that V-ATPase inhibitors induce the expression of COX-2 via NHE-dependent and -independent pathways.

Subjects

CELL lines; ADENOSINE triphosphatase; MESSENGER RNA; CYCLOOXYGENASES

Publication

FEBS Letters, 2007, Vol 581, Issue 24, p4633

ISSN

0014-5793

Publication type

Academic Journal

DOI

10.1016/j.febslet.2007.08.055