Background There are many causes of behavioral disorders in children investigating the causes and efforts to reduce them are useful for children. Methods This causal-comparative research is cross-sectional study among of the children born with assisted reproductive technology and natural fertility methods in the Afzalipour hospital in Kerman city. A sample of 30 children were born with assisted reproductive technology and 30 children were born with natural methods of fertility chooses randomly and two groups matched on the basis age, sex, education, father's occupation, and family members. For two groups completed Czech list revision Quay-Peterson and Data were analyzed by SPSS software version 16. Findings The results showed that differentiation between behavioral disorders (0.002) and its components, including attention-immaturity (0.008) and anxiety-recluse (0.000) in two groups were significant (increased in children born with infertility treatments), but between the elements of aggression (0.911) and conduct disorder (0.066) were not a significant difference. Conclusion This study showed that the kind of fertilization effective on behavioral disorders in children.