EMBRYO QUALITY AFTER OOCYTE VITRIFICATION.

Pareja, Sandra; Losada, Carolina; Fernandez, Jesus; Jimenez, Maria; Bou, Carmen; Pellicer, Antonio; Herrero, Leyre; Garcia-Velasco, Juan Antonio

Aim: Vitrification allows efficient and secure oocyte cryopreservation in patients who want to preserve their fertilization, in OHSS cases and in low responders. The aim of our study is to compare the quality of the embryos obtained from vitrified and fresh oocytes belonging to low responders which have accumulated mature oocytes during more than one cycle of stimulation, and joined them to fresh ones obtained in the last stimulation cycle, in order to have more embryos to select for transfer. Method: Retrospective analysis of 243 embryos belonging to 45 patients underwent ovarian puncture, from both fresh and thawed oocytes. The vitrification process was carry on following the Cryotop method, from January to September 2009. Average age of the patients was 36.3±4.2, MII accumulated 6.9±4.5 and embryos transferred 1.9±0.3. Preganancy rate was 60%, implantation rate 37.8%, and miscarriage rate 14.8%. None transfer was cancelled. Results: Retrospective analysis of 243 embryos belonging to 45 patients underwent ovarian puncture, from both fresh and thawed oocytes. The vitrification process was carry on following the Cryotop method, from January to September 2009. Average age of the patients was 36.3±4.2, MII accumulated 6.9±4.5 and embryos transferred 1.9±0.3. Preganancy rate was 60%, implantation rate 37.8%, and miscarriage rate 14.8%. None transfer was cancelled. Conclusion: With these preliminary results we can confirm that vitrification does not affect embryo development in low responder patients that accumulate oocytes from previous stimulation cycles. Despite of that, more studies are necessary to asses our observations.

CRYOPRESERVATION of organs, tissues, etc.; OVUM; CONCEPTION; MISCARRIAGE; PREGNANCY

Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2010, Vol 20, pS59

1472-6483

Academic Journal

10.1016/S1472-6483(10)62409-1