Results
Title

Recombinant glucocorticoid induced tumor necrosis factor receptor (rGITR) induces NOS in murine macrophage

Authors

Shin, Hyun-Hee; Lee, Moo-Hyung; Kim, Suk-Gi; Lee, Yun-Hwa; Kwon, Byoung S.; Choi, Hye-Seon

Abstract

Glucocorticoid induced tumor necrosis factor receptor (GITR) is a new member of the tumor necrosis factor–nerve growth factor receptor superfamily of which the function has not been well studied. The extracellular domain of GITR was produced in Escherichia coli and purified as a single band of predicted Mr of 18.0 kDa. GITR and GITR ligand were expressed constitutively on the surface of Raw 264.7 macrophage cell line and murine peritoneal macrophages. An extracellular domain of GITR can activate murine macrophages to express inducible nitric oxide synthase and to generate nitric oxide in a dose- and time-dependent manner.

Subjects

GLUCOCORTICOIDS; TUMOR necrosis factors

Publication

FEBS Letters, 2002, Vol 514, Issue 2/3, p275

ISSN

0014-5793

Publication type

Academic Journal

DOI

10.1016/S0014-5793(02)02379-7