microRNA-26b inhibits growth and cellular invasion of ovarian cancer cells by targeting estrogen receptor α.

He, Guna; Liu, Xiaomei; Liu, Lingyu

The current study set out to elucidate the mechanism of miR-26b in OC cell proliferation and EMT via suppression of ERα. Initial findings illustrated that miR-26b was poorly expressed in OC tissues and cells. On the other hand, over-expression of miR-26b exerted a diminishing effect on SKOV3 cell proliferation, migration, invasion and EMT, whereas silencing of miR-26b conferred an enhancing effect on CAOV3 cell proliferation, migration, invasion and EMT. Subsequently, with help from the TargetScan database, a dual-luciferase reporter gene assay was carried out to verify the targeting relation between miR-26b and ERα, which revealed that miR-26b could negatively modulate ERα. Furthermore, the in vivo experimentation illustrated that over-expression of miR-26b led to down-regulation of ERα and suppression OC tumor growth and EMT. Meanwhile, silencing of ERα inhibited OC cell proliferation, migration, invasion and EMT. In conclusion, our findings indicated that miR-26b inhibited OC cell proliferation and EMT via negative-modulation of ERα. This investigation may offer potential strategy for OC treatment.

CELL growth; OVARIAN cancer; INHIBITION of cellular proliferation; CELL proliferation; ESTROGEN receptors; REPORTER genes

3 Biotech, 2022, Vol 12, Issue 8, p1

2190-572X

Academic Journal

10.1007/s13205-022-03222-2