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- Title
Olfactory mucosa stem cells delivery via nasal route: a simple way for the treatment of Parkinson disease.
- Authors
Simorgh, Sara; Alizadeh, Rafieh; Shabani, Ronk; Karimzadeh, Fariba; Seidkhani, Elham; Majidpoor, Jamal; Moradi, Fatemeh; Kasbiyan, Hamidreza
- Abstract
Finding a simple and effective way for transferring cells to the brain lesion site with minimum side effects mounts a challenge in cell therapy. Cell delivery via nasal route using the bypassing the blood-brain barrier (BBB) property is a simple and non-invasive strategy without serious complications such as trauma. Therefore, it is a suitable technique to treat neurodegenerative disorders like Parkinson's disease (PD). Olfactory ectomesenchymal stem cells (OE-MSCs) located in the lamina propria of olfactory mucosa could be differentiated into dopaminergic neurons under in vitro and in vivo conditions. Thus, OE-MSCs represent a good source of Parkinson's stem cell–based therapy. In this research, we studied thirty male rats (n = 10 in each group) in three control (Ctl), lesion (LE), and intranasal administration (INA) groups to investigate the therapeutic effect of intranasal injection of OE-MSCs in the Parkinson's animal models. To do so, we examined the homing variation of OE-MSCs in different brain regions such as olfactory bulb (OB), cortex, striatum (Str), hippocampus (HPC), and substantia nigra (SN). The results of real-time PCR and immunohistochemistry (IHC) analysis showed the expression of dopaminergic neuron markers such as PITX3, PAX2, PAX5 (as dopaminergic neurons markers), tyrosine hydroxylase (TH), and dopamine transporter (DAT) 2 months after INA of 1 × 106 OE-MSCs. The results confirmed that IN OE-MSCs delivery into the central nervous system (CNS) was powerful enough to improve the behavioral functions in the animal models of PD.
- Subjects
STEM cells; THERAPEUTICS; PARKINSON'S disease; BLOOD-brain barrier; CENTRAL nervous system; DOPAMINERGIC neurons; NASAL mucosa
- Publication
Neurotoxicity Research, 2021, Vol 39, Issue 3, p598
- ISSN
1029-8428
- Publication type
Academic Journal
- DOI
10.1007/s12640-020-00290-1