Monolithic Material Prepared with Nanodiamond as Monomer for the Enrichment of β-Sitosterol in Edible Oil.

Cui, Beijiao; Liu, Haiyan; Yu, Huan; Pang, Xiaoya; Yan, Hongyuan; Bai, Ligai

In this paper, a hybrid monolithic column with modified nanodiamond and 1-dodecene as co-monomers was prepared and applied to the online enrichment and purification of β-sitosterol in edible oil. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the linear regression coefficient for β-sitosterol was 0.998; the limit of detection (LOD) and the limit of quantitation (LOQ) were 3.0 μg mL−1 and 10.0 μg mL−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for four edible oils were 2.33-6.58% and 3.24-6.94%, respectively. The recovery was in the range of 89.6-105.5%. The results showed that the hybrid monolithic column with high selectivity and good permeability were successfully used as online solid-phase extraction (SPE) column for determination of β-sitosterol in edible oil.

Food Analytical Methods, 2019, Vol 12, Issue 3, p697

1936-9751

Academic Journal

10.1007/s12161-018-1405-9