Results
Title

Investigation Cytotoxicity and Curcumin Release Behavior by Pyranopyrazole-TiO<sub>2</sub>@niosome Carrier for Breast Cancer Treatment.

Authors

Yeganeh, Faten Eshrati; Ghafuri, Hossein; Azizi, Mojtaba

Abstract

In the present study, we present a pyranopyrazole-TiO2 which is encapsulated with a niosome as nanocarrier for delivery of curcumin into breast cancer cells. Nanocarrier porous TiO2 is biocompatible and with a high specific surface area and a large pore volume and was used to carry pyranopyrazole, which has been reported as an anti-cancer. Niosome in the outer layer, helpful for loading curcumin into the niosomal layer, demonstrates a pH-dependent release and can be effective for cancer treatment. Entrapment efficiency of curcumin was found at 81.02% in carriers. The results of MTT and flow cytometry revealed that apoptosis is notably enhanced by loading curcumin on pyranopyrazole-TiO2@niosome. Also, there was high biocompatibility with MCF-10A, while exhibiting significant anti-cancer and anti-metastatic effects on MCF-7, whose cell viability was 38.79% in the loaded curcumin on carrier and was more than other samples even, than free curcumin (42.82%). Furthermore, the regulation of gene expression in cancer cells decreased the regulation of MMP-2 and MMP-9 genes and increased the expression of caspase-3 and caspase-9 genes. Finally, fluorescence activity in MCF-7 significantly increased after treatment with samples.

Subjects

GENETIC regulation; MEDICAL sciences; CELLULAR control mechanisms; CYTOTOXINS; TITANIUM dioxide

Publication

Applied Biochemistry & Biotechnology, 2024, Vol 196, Issue 12, p8530

ISSN

0273-2289

Publication type

Academic Journal

DOI

10.1007/s12010-024-04985-5